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Genetic Engineering

Episodes

Posted on September 2nd 2022 (over 2 years)

In this clip, Dr. George Church describes the pros and cons of animal research and how we could move beyond its use with advanced technologies.

Posted on September 2nd 2022 (over 2 years)

In this clip, Dr. George Church discusses how gene editing could uniquely improve human life in the future.

Posted on September 2nd 2022 (over 2 years)

In this clip, Dr. George Church discusses these findings and what they could mean for the future of public health.

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News & Publications

  • CAR-T, or chimeric antigen receptor T-cell therapy, is an immunotherapy approach that involves genetically modifying a person’s own T cells so they can recognize and target specific proteins on cancer cell surfaces, enhancing the immune system’s capacity to seek out and destroy malignant cells. CAR-T therapies have been successful against blood cancers, such as leukemia, lymphoma, or myeloma, but have largely failed with solid tumors. Now, a new study in mice demonstrates that CAR-T is effective against ovarian cancer, nearly doubling survival time.

    Researchers identified a unique carbohydrate present only on the surface of solid tumor cells, not healthy ones, and engineered CARs with a strong affinity for the carbohydrate. Then, they delivered the CAR-T therapy via intravenous injection to mice with ovarian cancer. Because ovarian cancer treatments delivered directly into the abdominal area are particularly effective, they also administered the CAR-T therapy into the animals' abdomens.

    They found that the CAR-equipped T cells effectively located and eliminated the cancer cells, promoting tumor shrinkage or elimination with just one dose. The genetically engineered cells maintained their effectiveness for several months, with no evidence of toxicity or adverse effects. Intravenous injection of CAR-T cells increased survival to 145 days, but direct delivery into the animals' abdomens extended survival to 270 days.

    These findings demonstrate that modified CAR-T cells show promise as a potential treatment for ovarian cancer and other solid tumors. Future studies are needed to assess the treatment’s effectiveness in humans. Learn more about genetic engineering in this episode featuring Dr. George Church.

  • Gene therapy treats transfusion-dependent beta-thalassemia.

    Gene therapy is a technique that modifies a person’s genes to prevent, treat, or cure a disease. Gene therapies work by a variety of mechanisms, including replacing a disease-causing gene with a healthy version of the gene, inactivating a disease-causing gene, or introducing a new or modified gene or genes into the body. Findings from a phase 3 clinical trial suggest that beti-cel gene therapy successfully treats transfusion-dependent beta-thalassemia.

    Beti-cel is a gene therapy that works by adding a modified form of the beta-globin gene into a recipient’s own blood cell-producing stem cells. This process relies on transduction, the process by which foreign DNA is introduced into a cell by a virus or viral vector. Beti-cel therapy is designed to facilitate the production of normal, healthy hemoglobin and obviate the need for transfusions.

    Thalassemia is a class of inherited blood disorders that affect the genes for hemoglobin, the oxygen-carrying component of the red blood cells. Hemoglobin, which consists of two proteins (alpha and beta) becomes incorporated into red blood cells during their maturation. Insufficient production of either the alpha or beta proteins due to genetic defect impairs oxygen transport via the red blood cells, inducing anemia and often necessitating blood transfusions.

    The study involved 22 people (ages 4 to 34 years) with transfusion-dependent beta-thalassemia. Prior to receiving the gene therapy, each of the participants underwent chemotherapy to temporarily halt their red blood cell production. The investigators administered beti-cel intravenously and monitored the recipients for an average of 30 months.

    They found that 20 of the 22 participants, including six who were under the age of 12 years, no longer required blood transfusions after receiving the gene therapy, typically within one month of administration. Four of the participants experienced adverse events related to the gene therapy, but most events were mild except for one event in which the participant developed thrombocytopenia, a condition in which blood platelet levels are too low.

    These findings suggest that beti-cel gene therapy successfully treats transfusion-dependent beta-thalassemia and opens the door for more gene therapy strategies and applications in the future. Learn more about gene therapy in this episode featuring world-renowned geneticist Dr. George Church.